Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.7

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.1250	0.0335	0.1563
	B	25.00	0.1304	0.1244	0.1204
	C	8.33	0.1379		0.1213
	D	2.78	0.0679	0.0618	0.0617
	E	0.93	0.02655	0.02655	0.02600
	F	0.31	0.01050	0.01080	0.01065
	G	0.10	0.00345	0.00335	0.00350
	H	0.03	0.001300		0.001400

Yield (mg/mL): 1.7     Dilution Factor:  100×

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by kavyashankar and last updated on 2023-11-29 14:57:17.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.0005000	0.000500
	B	48.3	0.0005000	0.001300	0.0008000
	C	45.7	0.000900	0.001000	0.001000
	D	42.4	0.04950	0.04340	0.06520
	E	37.7	0.1630	0.1682	0.1590
	F	33.6	0.1242	0.1875	0.1711
	G	31.3	0.1749	0.1625	0.1804
	H	30.0	0.1806	0.1900	0.2196

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by kavyashankar and last updated on 2023-11-29 15:08:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.