D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H178R

Expression Data

Yield (mg/mL): 0.091

Kinetic Assay Data

Plate 20201217-tali2

**slope of H178R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.00616242	0.00536848
B	18.75	0.00442242	0.00450485
C	4.69	0.00349091	0.00271879	0.00274303
D	1.17	0.00133515	0.00118182	0.0010097
E	0.29	0.000324848	0.00024303	0.0003
F	0.07	0.000129697	0.000123636	0.000165455
G	0.02	9.88E-05	0	4.48E-05
H	0.00	5.76E-05	2.85E-05	3.94E-05

Yield / A_{280 nm}: 0.091 Dilution Factor: 10×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0102655	0.00766848
B	18.75	0.00849818	0.00768848
C	4.69	0.0049703	0.00447758
D	1.17	0.00229455	0.00184606
E	0.29	0.000653939	0.000593939
F	0.07	0.000184242	0.000178788
G	0.02	1.37E-04		0.000159394
H	0.00	1.70E-05		2.30E-05

Yield / A_{280 nm}: 0.102 Dilution Factor: 50×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.