Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.5

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3034	0.3265	0.3365
	B	25.00	0.2429	0.2700	0.2605
	C	8.33	0.2028	0.2426	0.2283
	D	2.78	0.1896	0.1578	0.1641
	E	0.93	0.12050	0.07395	0.09180
	F	0.31	0.04890	0.02885	0.04095
	G	0.10	0.02400	0.01120	0.01795
	H	0.03	0.000200	0.000150	0.000300

Yield (mg/mL): 1.5     Dilution Factor:  100×

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by Beni and last updated on 2023-11-29 14:50:49.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00040	0.0005000	0.000500
	B	48.3	0.0004000	0.000600	0
	C	45.7		0.001100	0.001100
	D	42.4	0.13430	0.12890	0.13800
	E	37.7	0.2760	0.2847	0.2653
	F	33.6	0.5940	0.6173	0.5518
	G	31.3
	H	30.0

Download these data

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by Beni and last updated on 2023-12-21 18:33:17.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.