D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q392N

Expression Data

Yield (mg/mL): 0.471

Kinetic Assay Data

Plate 20201217-tali2

**slope of Q392N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.0225345	0.0191964
B	18.75		0.0155309	0.0135788
C	4.69	0.0152406	0.0104327	0.00824
D	1.17		0.00386788	0.00318909
E	0.29		0.00123333	0.00123455
F	0.07		0.000372121	0.000354545
G	0.02	0.000150303	1.12E-04	1.24E-04
H	0.00		0	0

Yield / A_{280 nm}: 0.471 Dilution Factor: 100×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0102655	0.00766848
B	18.75	0.00849818	0.00768848
C	4.69	0.0049703	0.00447758
D	1.17	0.00229455	0.00184606
E	0.29	0.000653939	0.000593939
F	0.07	0.000184242	0.000178788
G	0.02	1.37E-04		0.000159394
H	0.00	1.70E-05		2.30E-05

Yield / A_{280 nm}: 0.102 Dilution Factor: 50×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.