D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S169N

Expression Data

Yield (mg/mL): 0.277

Kinetic Assay Data

Plate 20201217-alex

**slope of S169N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.000613333	0.000658182	0.00051697
B	18.75	0.00042303	0.000274545	0.000115152
C	4.69	0.000335758	0.000362424
D	1.17	7.39E-05		0.00006
E	0.29	3.21E-05		2.97E-05
F	0.07	6.18E-05	0	2.97E-05
G	0.02	1.24E-04	6.73E-05	8.73E-05
H	0.00	0	0	0

Yield / A_{280 nm}: 0.277 Dilution Factor: 10×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0118679	0.0116933	0.0100139
B	18.75	7.23E-03	0.00763152	6.20E-03
C	4.69	5.29E-03	5.77E-03
D	1.17	4.11E-03	4.31E-03	3.40E-03
E	0.29		1.59E-03	1.62E-03
F	0.07	4.50E-04	4.82E-04
G	0.02	2.56E-04	1.78E-04	3.31E-04
H	0.00	1.15E-04		1.30E-04

Yield / A_{280 nm}: 0.275 Dilution Factor: 100×

Protein purified on 2001.01.20 and assayed on 2001.01.20.
Data uploaded by vater and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.