D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H178W

Expression Data

Yield (mg/mL): 1.32

Kinetic Assay Data

**slope of H178W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.55E-01	2.72E-01	2.77E-01
B	25.00	2.01E-01	2.12E-01	2.13E-01
C	8.33	1.43E-01	1.59E-01	1.65E-01
D	2.78	1.28E-01	1.21E-01	1.52E-01
E	0.93	4.92E-02	5.32E-02	6.63E-02
F	0.31	1.84E-02	0	2.52E-02
G	0.10		7.75E-03	6.60E-03
H	0.03	2.00E-04		1.50E-04

Yield (mg/mL): 1.32 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by MutationJon2023 and last updated on 2023-12-06 12:49:21.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.99E-01	1.87E-01	1.92E-01
B	25.00	1.54E-01	1.11E-01	1.56E-01
C	8.33	1.14E-01	1.12E-01	1.01E-01
D	2.78	9.59E-02	1.04E-01	7.92E-02
E	0.93	2.95E-02	2.85E-02	2.97E-02
F	0.31	1.15E-02	1.07E-02	1.46E-02
G	0.10	5.35E-03	4.00E-03	5.70E-03
H	0.03	0	2.00E-04	3.50E-04

Yield (mg/mL): 0.863 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by MutationJon2023 and last updated on 2023-12-06 17:01:06.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of H178W A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		2.00E-04	2.50E-04
B	48.3	2.50E-04	2.50E-04
C	45.7	4.00E-04	3.50E-04	4.50E-04
D	42.4	1.34E-02	2.38E-02	1.94E-02
E	37.7	3.92E-02		3.79E-02
F	33.6	4.65E-02	4.00E-02	4.51E-02
G	31.3		4.55E-02	4.74E-02
H	30.0	4.99E-02		4.15E-02

Protein purified on 11/8/23 and assayed on 11/15/23.
Data uploaded by ejaneckova and last updated on 2023-12-17 14:40:35.

Design Data

Rosetta Score for H178W: 20.167

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Eva Janeckova: <ejaneckova@miracosta.edu>.