D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N160C

Expression Data

Yield (mg/mL): 4.07

Kinetic Assay Data

**slope of N160C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.99E-01	6.28E-01	5.99E-01
B	25.00	4.73E-01	4.30E-01	4.57E-01
C	8.33	3.77E-01	3.59E-01	3.51E-01
D	2.78	2.69E-01	2.16E-01	2.34E-01
E	0.93	1.25E-01	1.38E-01	1.19E-01
F	0.31	1.52E-02	1.37E-02	1.58E-02
G	0.10	0	0
H	0.03

Yield (mg/mL): 4.07 Dilution Factor: 100×

Protein purified on 10/8/23 and assayed on 10/9/23.
Data uploaded by DimitriosZannis and last updated on 2023-12-20 14:10:12.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.21E-01	4.45E-01	4.85E-01
B	25.00	3.84E-01	3.94E-01	3.72E-01
C	8.33	2.60E-01	2.73E-01	2.44E-01
D	2.78	1.56E-01	1.62E-01	1.47E-01
E	0.93	7.32E-02	7.44E-02	6.10E-02
F	0.31	7.80E-03	9.40E-03	8.10E-03
G	0.10	6.00E-04	6.00E-04
H	0.03		4.00E-04	4.00E-04

Yield (mg/mL): 4.094 Dilution Factor: 100×

Protein purified on 10/8/23 and assayed on 10/9/23.
Data uploaded by DimitriosZannis and last updated on 2023-12-20 11:37:16.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.