D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N160S

Expression Data

Yield (mg/mL): 3.61

Kinetic Assay Data

**slope of N160S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.93E-01	3.97E-01	4.14E-01
B	25.00	3.19E-01	3.15E-01	3.12E-01
C	8.33	2.14E-01	2.33E-01	2.19E-01
D	2.78	1.33E-01	1.33E-01	1.57E-01
E	0.93	6.49E-02	6.59E-02	6.17E-02
F	0.31	8.10E-03	7.30E-03	7.70E-03
G	0.10	0	0
H	0.03	0	0	0

Yield (mg/mL): 3.61 Dilution Factor: 100×

Protein purified on 10/8/23 and assayed on 10/9/23.
Data uploaded by DimitriosZannis and last updated on 2023-12-20 14:11:21.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.21E-01	4.45E-01	4.85E-01
B	25.00	3.84E-01	3.94E-01	3.72E-01
C	8.33	2.60E-01	2.73E-01	2.44E-01
D	2.78	1.56E-01	1.62E-01	1.47E-01
E	0.93	7.32E-02	7.44E-02	6.10E-02
F	0.31	7.80E-03	9.40E-03	8.10E-03
G	0.10	6.00E-04	6.00E-04
H	0.03		4.00E-04	4.00E-04

Yield (mg/mL): 4.094 Dilution Factor: 100×

Protein purified on 10/8/23 and assayed on 10/9/23.
Data uploaded by DimitriosZannis and last updated on 2023-12-20 11:37:16.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.