Raw Data for BglB Variant N404C

Expression Data

Yield (mg/mL): 1.5495

Kinetic Assay Data

slope of N404C A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.54E-02	2.71E-02	2.89E-02
	B	25.00	2.46E-02	1.90E-02	1.83E-02
	C	8.33		1.08E-02	1.00E-02
	D	2.78		4.20E-03	4.35E-03
	E	0.93	2.70E-03	1.65E-03	2.00E-03
	F	0.31		8.00E-04	7.50E-04
	G	0.10	4.50E-04	4.00E-04	4.00E-04
	H	0.03		1.50E-04	1.50E-04

Yield (mg/mL): 1.5495     Dilution Factor:  100×

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Protein purified on 11/9/2023 and assayed on 11/16/2023.
Data uploaded by AV and last updated on 2023-12-07 12:22:24.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.87E-01	3.66E-01	3.68E-01
	B	25.00	2.69E-01	2.80E-01	2.75E-01
	C	8.33
	D	2.78		1.43E-01	1.38E-01
	E	0.93	6.53E-02	6.48E-02	6.30E-02
	F	0.31	2.34E-02	2.32E-02	2.32E-02
	G	0.10	8.60E-03	8.10E-03	9.50E-03
	H	0.03	4.00E-04	3.50E-04

Yield (mg/mL): 1.491     Dilution Factor:  100×

Download these data

Protein purified on 11/9/23 and assayed on 11/16/23.
Data uploaded by Leopreciado and last updated on 2023-12-07 13:02:18.

Thermostability Assay Data

slope of N404C A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	0.00E+00
	B	48.3	3.00E-03	2.45E-03
	C	45.7	1.80E-03	3.30E-03	6.00E-04
	D	42.4	1.84E-02	1.89E-02
	E	37.7	2.01E-02	2.30E-02
	F	33.6	2.25E-02	2.17E-02
	G	31.3	2.10E-02	2.30E-02
	H	30.0	2.05E-02	2.11E-02

Download these data

Protein purified on 11/16/23 and assayed on 11/18/23.
Data uploaded by brendaamezquita and last updated on 2023-12-07 12:38:32.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.