Raw Data for BglB Variant A306S

Expression Data

Yield (mg/mL): 1.8905

Kinetic Assay Data

slope of A306S A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.83E-01	2.56E-01	2.59E-01
	B	25.00	1.99E-01	2.02E-01	2.46E-01
	C	8.33	1.41E-01	1.44E-01	1.50E-01
	D	2.78	7.96E-02	8.26E-02	8.68E-02
	E	0.93	3.39E-02	3.19E-02	3.87E-02
	F	0.31	1.33E-02	1.21E-02	1.34E-02
	G	0.10	4.05E-03	4.20E-03	4.65E-03
	H	0.03		2.00E-04	2.00E-04

Yield (mg/mL): 1.8905     Dilution Factor:  100×

Download these data

Protein purified on 11/9/2023 and assayed on 11/16/2023.
Data uploaded by TiffanyQ and last updated on 2023-12-07 12:48:27.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.87E-01	3.66E-01	3.68E-01
	B	25.00	2.69E-01	2.80E-01	2.75E-01
	C	8.33
	D	2.78		1.43E-01	1.38E-01
	E	0.93	6.53E-02	6.48E-02	6.30E-02
	F	0.31	2.34E-02	2.32E-02	2.32E-02
	G	0.10	8.60E-03	8.10E-03	9.50E-03
	H	0.03	4.00E-04	3.50E-04

Yield (mg/mL): 1.491     Dilution Factor:  100×

Download these data

Protein purified on 11/9/23 and assayed on 11/16/23.
Data uploaded by Leopreciado and last updated on 2023-12-07 13:02:18.

Thermostability Assay Data

slope of A306S A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.50E-04		2.50E-04
	B	48.3		0.00E+00	0.00E+00
	C	45.7	6.00E-04	6.50E-04	5.50E-04
	D	42.4		4.73E-02	4.65E-02
	E	37.7	2.58E-01	2.59E-01	2.58E-01
	F	33.6	2.75E-01	2.84E-01	2.72E-01
	G	31.3	2.72E-01	2.74E-01	2.72E-01
	H	30.0	2.68E-01	2.73E-01	2.93E-01

Download these data

Protein purified on 11/9/2023 and assayed on 11/18/2023.
Data uploaded by Saavi and last updated on 2023-12-07 12:35:23.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.