Raw Data for BglB Variant A306K

Expression Data

SDS-PAGE
 1.554

Kinetic Assay Data

slope of A306K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 3.41E-01 3.22E-01 3.17E-01
B 25.00 2.39E-01 2.49E-01 2.51E-01
C 8.33 1.90E-01 1.88E-01 1.92E-01
D 2.78 1.49E-01 1.38E-01 1.28E-01
E 0.93 6.11E-02 6.52E-02 5.49E-02
F 0.31 2.14E-02 2.26E-02 2.04E-02
G 0.10 8.15E-03 7.75E-03 9.30E-03
H 0.03 3.00E-04 2.50E-04 1.50E-04
 1.554      100×

Protein purified on 11/9/23 and assayed on 11/16/23.
Data uploaded by NatalyaPhillips and last updated on 2023-12-07 12:29:35.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.3191 0.318 0.3013
B 25.00 0.2246 0.2261 0.22
C 8.33 0.1997 0.1651 0.172
D 2.78 0.1281 0.1511 0.1249
E 0.93 0.0561 0.0573 0.05295
F 0.31 0.0215 0.0214 0.02035
G 0.10 0.0073 0.0074 0.0063
H 0.03 0.00015 0 0.0002
 1.441      100×

Protein purified on 11/9/2023 and assayed on 11/16/2023.
Data uploaded by AndreaskZImmermann and last updated on 2023-12-07 12:51:03.


Thermostability Assay Data

slope of A306K A420 nm (min−1)
T (°C) 1 2 3
A 30.0 0.2612 0.2489 0.253
B 31.3 0.2489 0.2491 0.2561
C 33.6 0.2481 0.2463 0.2515
D 37.7 0.2406 0.2416 0.2405
E 42.4 0.0583 0.04825 0.04055
F 45.7 0.0222 0.0205
G 48.3 0.00935 0.0002 0.0164
H 50.0 0.00085 0.0002 0.0018

Protein purified on 11/9/23 and assayed on 11/18/23.
Data uploaded by NatalyaPhillips and last updated on 2023-12-07 12:49:27.


Reference Data for WT

slope of A306K A420 nm (min−1)
T (°C) 1 2 3
A 30.0 0.2612 0.2489 0.253
B 31.3 0.2489 0.2491 0.2561
C 33.6 0.2481 0.2463 0.2515
D 37.7 0.2406 0.2416 0.2405
E 42.4 0.0583 0.04825 0.04055
F 45.7 0.0222 0.0205
G 48.3 0.00935 0.0002 0.0164
H 50.0 0.00085 0.0002 0.0018

Protein purified on 11/9/2023 and assayed on 11/18/2023.
Data uploaded by AndreaskZImmermann and last updated on 2023-12-07 12:34:54.


Design Data

-1.495

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.