Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.441

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.3191	0.318	0.3013
	B	25.00	0.2246	0.2261	0.22
	C	8.33	0.1997	0.1651	0.172
	D	2.78	0.1281	0.1511	0.1249
	E	0.93	0.0561	0.0573	0.05295
	F	0.31	0.0215	0.0214	0.02035
	G	0.10	0.0073	0.0074	0.0063
	H	0.03	0.00015	0	0.0002

Yield (mg/mL): 1.441     Dilution Factor:  100×

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Protein purified on 11/9/2023 and assayed on 11/16/2023.
Data uploaded by AndreaskZImmermann and last updated on 2023-12-07 12:51:03.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	30.0	0.2612	0.2489	0.253
	B	31.3	0.2489	0.2491	0.2561
	C	33.6	0.2481	0.2463	0.2515
	D	37.7	0.2406	0.2416	0.2405
	E	42.4	0.0583	0.04825	0.04055
	F	45.7	0.0222		0.0205
	G	48.3	0.00935	0.0002	0.0164
	H	50.0	0.00085	0.0002	0.0018

Download these data

Protein purified on 11/9/2023 and assayed on 11/18/2023.
Data uploaded by AndreaskZImmermann and last updated on 2023-12-07 12:34:54.

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.