D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H178K

Expression Data

Yield (mg/mL): 0.672

Kinetic Assay Data

**slope of H178K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.38E-02	3.52E-02	3.20E-02
B	25.00	2.07E-02	2.40E-02	2.23E-02
C	8.33	9.75E-03	1.18E-02	1.11E-02
D	2.78	4.90E-03	4.80E-03	5.10E-03
E	0.93	1.90E-03	1.95E-03	2.00E-03
F	0.31	7.50E-04	8.00E-04	9.00E-04
G	0.10	4.00E-04	4.50E-04	3.50E-04
H	0.03	2.50E-04	2.50E-04	2.50E-04

Yield (mg/mL): 0.672 Dilution Factor: 10×

Protein purified on 11/15/23 and assayed on 11/17/23.
Data uploaded by mvlouie and last updated on 2023-12-07 14:49:50.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.09E-01	3.13E-01	3.09E-01
B	25.00	2.49E-01	2.41E-01	2.37E-01
C	8.33	1.79E-01	1.74E-01	1.55E-01
D	2.78	9.94E-02	1.01E-01	9.95E-02
E	0.93	4.55E-02	4.53E-02	4.42E-02
F	0.31	1.70E-02	1.65E-02	1.66E-02
G	0.10	6.10E-03	5.90E-03	5.75E-03
H	0.03	2.35E-03	2.10E-03	2.20E-03

Yield (mg/mL): 3.678 Dilution Factor: 100×

Protein purified on 11/15/23 and assayed on 11/17/23.
Data uploaded by mvlouie and last updated on 2023-11-28 12:17:38.

Design Data

Rosetta Score for H178K: 8.901

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.