D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R239L

Expression Data

Yield (mg/mL): 2.79008E-5

Kinetic Assay Data

**slope of R239L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.73E-01	2.53E-01	2.43E-01
B	25.00	2.42E-01	2.14E-01	2.12E-01
C	8.33	2.02E-01	1.93E-01	1.98E-01
D	2.78	1.09E-01	1.15E-01	9.93E-02
E	0.93	4.56E-02	4.58E-02	3.94E-02
F	0.31	1.57E-02	1.53E-02	1.36E-02
G	0.10	6.52E-03	7.24E-03	6.62E-03
H	0.03	0		0

Yield (m): 2.79008E-5 Dilution Factor: 100×

Protein purified on 2/28/24 and assayed on 3/14/24.
Data uploaded by zizi197 and last updated on 2024-04-11 07:30:34.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.76E-01	2.90E-01	2.76E-01
B	25.00	2.19E-01	2.25E-01	2.22E-01
C	8.33	2.03E-01	1.96E-01	1.93E-01
D	2.78	1.16E-01	1.18E-01	1.26E-01
E	0.93	5.84E-02	5.35E-02	5.89E-02
F	0.31	2.34E-02	2.51E-02	2.54E-02
G	0.10	1.10E-02	1.13E-02	1.15E-02
H	0.03	0	0	0

Yield (mg/mL): 1.0257 Dilution Factor: 100×

Protein purified on 2/28/24 and assayed on 3/14/24.
Data uploaded by zizi197 and last updated on 2024-04-18 07:23:30.

Thermostability Assay Data

**slope of R239L A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	1.87E-01	2.00E-01
B	30.5	1.58E-01	1.98E-01
C	31.7	1.89E-01	1.71E-01
D	33.2	0.14783	0.13697
E	35.4	0.11514	0.11017
F	38.3	0.043759	0.035897
G	41.7	0.0047586	0.0049655
H	44.5	0.0026897	0.0024828
I	46.8	0.0027931	0
J	48.3	0	0
K	49.5	0.0027931	0
L	50.0	0	0

Protein purified on 2/28/24 and assayed on 3/21/24.
Data uploaded by zizi197 and last updated on 2024-04-18 07:03:16.

Reference Data for WT

**slope of R239L A_{420 nm} (min⁻¹)**
	T (°C)	1	2
A	30.0	2.68E-01	2.43E-01
B	30.5	2.24E-01	2.30E-01
C	31.7	2.60E-01	2.47E-01
D	33.2	0.22397	0.2219
E	35.4	0.20503	0.23079
F	38.3	0.24248	0.21724
G	41.7	0.087103	0.097345
H	44.5	0.0058966	0.0066207
I	46.8	0	0.0028966
J	48.3	0	0.0024828
K	49.5	0	0
L	50.0	0	0

Protein purified on 2/28/24 and assayed on 3/21/24.
Data uploaded by zizi197 and last updated on 2024-04-11 07:12:47.

Design Data

Rosetta Score for R239L: -0.276

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.