D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325Q

Expression Data

Yield (mg/mL): 2.613

Kinetic Assay Data

**slope of W325Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0091	0.0091	0.0113
B	25.00	0.0142	0.0099	0.0119
C	8.33	0.0111	0.0117	0.0098
D	2.78	0.0077	0.0083	0.0094
E	0.93	0.00415	0.00560	0.00580
F	0.31	0.00250	0.00275	0.00355
G	0.10	0.000750	0.001150	0.001500
H	0.03		0	0

Yield (mg/mL): 2.613 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/14/2023.
Data uploaded by Ixchel and last updated on 2024-01-22 01:33:04.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1280	0.1270	0.1260
B	25.00	0.1190	0.1030	0.1070
C	8.33	0.1050	0.0854	0.0831
D	2.78	0.0499		0.0467
E	0.93	0.02050	0.02830	0.02340
F	0.31	0.01100	0.01400
G	0.10	0.003050	0.005500	0.000450
H	0.03	0.000500	0.000450

Yield (mg/mL): 1.7 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/14/2023.
Data uploaded by Ixchel and last updated on 2024-01-17 15:40:10.

Design Data

Rosetta Score for W325Q: 3.904

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.