Raw Data for BglB Variant H315N

Expression Data

SDS-PAGE
 0.8958

Kinetic Assay Data

slope of H315N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.48E-01 1.48E-01 1.40E-01
B 25.00 1.28E-01 1.28E-01 1.27E-01
C 8.33 1.21E-01 1.21E-01 1.17E-01
D 2.78 8.37E-02 8.02E-02
E 0.93 4.82E-02 4.49E-02 5.05E-02
F 0.31 2.78E-02 3.78E-02 3.59E-02
G 0.10 2.47E-02 2.60E-02 2.57E-02
H 0.03 1.82E-02 2.18E-02 1.84E-02
 0.8958      100×

Protein purified on 1/15/2024 and assayed on 1/17/2024.
Data uploaded by storquato and last updated on 2024-02-20 13:20:36.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.91E-02 1.08E-01 1.12E-01
B 25.00 9.80E-02 1.08E-01 1.06E-01
C 8.33 9.80E-02 9.06E-02
D 2.78 5.88E-02 8.01E-02 5.79E-02
E 0.93 3.59E-02 4.56E-02 3.79E-02
F 0.31 2.85E-02 2.67E-02 3.26E-02
G 0.10 2.15E-02 2.33E-02 2.77E-02
H 0.03 1.62E-02 1.88E-02 1.92E-02
 0.633      100×

Protein purified on 1/15/2024 and assayed on 1/17/2024.
Data uploaded by storquato and last updated on 2024-02-24 14:49:07.


Thermostability Assay Data

slope of H315N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00 0.00E+00
B 48.7 0.00E+00 0.00E+00 0.00E+00
C 46.2 0.00E+00 0.00E+00 0.00E+00
D 42.3 1.96E-02 1.42E-02 2.89E-02
E 37.7 9.05E-02 8.56E-02 8.29E-02
F 33.9 9.23E-02 8.85E-02 9.73E-02
G 31.4 9.15E-02 9.05E-02 9.76E-02
H 30.0 9.13E-02 9.45E-02 1.01E-01

Protein purified on 1/15/2024 and assayed on 1/18/2024.
Data uploaded by storquato and last updated on 2024-03-29 13:37:26.


Reference Data for WT

slope of H315N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00 0.00E+00
B 48.7 0.00E+00 0.00E+00 0.00E+00
C 46.2 0.00E+00 0.00E+00 0.00E+00
D 42.3 1.83E-02 1.90E-02 1.88E-02
E 37.7 5.10E-02 5.54E-02 5.56E-02
F 33.9 6.04E-02 6.04E-02 6.11E-02
G 31.4 5.96E-02 6.14E-02 6.11E-02
H 30.0 5.99E-02 6.09E-02 6.21E-02

Protein purified on 1/15/2024 and assayed on 1/18/2024.
Data uploaded by storquato and last updated on 2024-03-29 13:30:43.


Design Data

-1.52

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.