Raw Data for BglB Variant H379Y

Expression Data

SDS-PAGE
 0.5675

Kinetic Assay Data

slope of H379Y A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 7.06E-02 6.13E-02 6.59E-02
B 25.00 6.33E-02 6.20E-02
C 8.33 5.43E-02 5.33E-02
D 2.78 4.84E-02 4.57E-02 4.79E-02
E 0.93 3.56E-02 3.49E-02
F 0.31 3.22E-02 2.96E-02 3.24E-02
G 0.10 2.96E-02 2.18E-02 3.35E-02
H 0.03 2.06E-02 2.07E-02 2.10E-02
 0.5675      100×

Protein purified on 1/15/2024 and assayed on 1/17/2024.
Data uploaded by storquato and last updated on 2024-02-20 13:40:36.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.91E-02 1.08E-01 1.12E-01
B 25.00 9.80E-02 1.08E-01 1.06E-01
C 8.33 9.80E-02 9.06E-02
D 2.78 5.88E-02 8.01E-02 5.79E-02
E 0.93 3.59E-02 4.56E-02 3.79E-02
F 0.31 2.85E-02 2.67E-02 3.26E-02
G 0.10 2.15E-02 2.33E-02 2.77E-02
H 0.03 1.62E-02 1.88E-02 1.92E-02
 0.633      100×

Protein purified on 1/15/2024 and assayed on 1/17/2024.
Data uploaded by storquato and last updated on 2024-02-24 14:49:07.


Thermostability Assay Data

slope of H379Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00 0.00E+00
B 48.7 0.00E+00 0.00E+00 0.00E+00
C 46.2 0.00E+00 0.00E+00 0.00E+00
D 42.3 2.18E-03 2.68E-04 1.50E-03
E 37.7 8.50E-03 8.47E-03 9.60E-03
F 33.9 9.56E-03 1.10E-02 1.06E-02
G 31.4 1.02E-02 9.90E-03 1.16E-02
H 30.0 1.04E-02 1.10E-02 1.23E-02

Protein purified on 1/15/2024 and assayed on 1/18/2024.
Data uploaded by storquato and last updated on 2024-03-29 13:38:46.


Reference Data for WT

slope of H379Y A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00 0.00E+00
B 48.7 0.00E+00 0.00E+00 0.00E+00
C 46.2 0.00E+00 0.00E+00 0.00E+00
D 42.3 1.83E-02 1.90E-02 1.88E-02
E 37.7 5.10E-02 5.54E-02 5.56E-02
F 33.9 6.04E-02 6.04E-02 6.11E-02
G 31.4 5.96E-02 6.14E-02 6.11E-02
H 30.0 5.99E-02 6.09E-02 6.21E-02

Protein purified on 1/15/2024 and assayed on 1/18/2024.
Data uploaded by storquato and last updated on 2024-03-29 13:30:43.


Design Data

-0.904

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.