D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T116S

Expression Data

Yield (mg/mL): 1.608

Kinetic Assay Data

**slope of T116S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.07615	0.0656	0.06825
B	25.00	0.0663	0.0547	0.05025
C	8.33	0.05785	0.0509	0.03945
D	2.78	0.03125	0.0331	0.0312
E	0.93	0.02545	0.02245	0.02125
F	0.31	0.01675	0.01555	0.012
G	0.10	0.0095	0.0113	0.0069
H	0.03	0.00035	0.0003	0.00035

Yield (mg/mL): 1.608 Dilution Factor: 100×

Protein purified on 1/23/24 and assayed on 1/23/24.
Data uploaded by enniferj and last updated on 2024-03-27 22:56:18.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.3694	0.3505
B	25.00		0.2409	0.2354
C	8.33	0.1146	0.2249	0.1913
D	2.78	0.1066	0.1344	0.1738
E	0.93	0.08875	0.1004	0.075
F	0.31	0.06365	0.07885	0.0643
G	0.10	0.03455	0.0292	0.02515
H	0.03	0.00035	0.0003	0.00035

Yield (mg/mL): 2.684 Dilution Factor: 100×

Protein purified on 1/23/24 and assayed on 1/23/24.
Data uploaded by enniferj and last updated on 2024-03-27 19:11:20.

Design Data

Rosetta Score for T116S: 4.001

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.