D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T296S

Expression Data

Yield (mg/mL): 2.462

Kinetic Assay Data

**slope of T296S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.2383	0.239
B	25.00		0.1647	0.1697
C	8.33	0.1146	0.1289	0.121
D	2.78	0.0809	0.1198	0.1072
E	0.93	0.05415	0.05585	0.05545
F	0.31	0.0238	0.0287	0.0297
G	0.10	0.01635	0.01905	0.02075
H	0.03	0		0

Yield (mg/mL): 2.462 Dilution Factor: 100×

Protein purified on 1/23/24 and assayed on 1/23/24.
Data uploaded by enniferj and last updated on 2024-03-27 23:00:19.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.3694	0.3505
B	25.00		0.2409	0.2354
C	8.33	0.1146	0.2249	0.1913
D	2.78	0.1066	0.1344	0.1738
E	0.93	0.08875	0.1004	0.075
F	0.31	0.06365	0.07885	0.0643
G	0.10	0.03455	0.0292	0.02515
H	0.03	0.00035	0.0003	0.00035

Yield (mg/mL): 2.684 Dilution Factor: 100×

Protein purified on 1/23/24 and assayed on 1/23/24.
Data uploaded by enniferj and last updated on 2024-03-27 19:11:20.

Design Data

Rosetta Score for T296S: 0.445

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.