D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant P429A

Expression Data

Yield (mg/mL): 2.506

Kinetic Assay Data

**slope of P429A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.32E-01	1.97E-01	2.12E-01
B	25.00	1.81E-01	1.85E-01
C	8.33	1.09E-01	8.15E-02	1.14E-01
D	2.78	6.11E-02	6.40E-02	5.73E-02
E	0.93	2.44E-02	2.61E-02	2.31E-02
F	0.31	9.05E-03	9.55E-03
G	0.10	3.05E-03	3.20E-03	2.95E-03
H	0.03	1.10E-03	1.15E-03	1.10E-03

Yield (mg/mL): 2.506 Dilution Factor: 100×

Protein purified on 1/24/24 and assayed on 1/31/24.
Data uploaded by 22Tristan22 and last updated on 2024-02-13 16:51:54.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		3.72E-01	3.71E-01
B	25.00	3.14E-01	2.82E-01	2.97E-01
C	8.33		1.79E-01	2.02E-01
D	2.78	1.18E-01	9.01E-02	1.00E-01
E	0.93	4.64E-02	3.71E-02	4.17E-02
F	0.31	1.57E-02	1.33E-02	1.49E-02
G	0.10	5.10E-03	4.55E-03	5.05E-03
H	0.03		1.75E-03	1.85E-03

Yield (mg/mL): 1.068 Dilution Factor: 100×

Protein purified on 1/24/24 and assayed on 1/31/24.
Data uploaded by 22Tristan22 and last updated on 2024-02-21 18:11:23.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.