Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.633

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	9.91E-02	1.08E-01	1.12E-01
	B	25.00	9.80E-02	1.08E-01	1.06E-01
	C	8.33		9.80E-02	9.06E-02
	D	2.78	5.88E-02	8.01E-02	5.79E-02
	E	0.93	3.59E-02	4.56E-02	3.79E-02
	F	0.31	2.85E-02	2.67E-02	3.26E-02
	G	0.10	2.15E-02	2.33E-02	2.77E-02
	H	0.03	1.62E-02	1.88E-02	1.92E-02

Yield (mg/mL): 0.633     Dilution Factor:  100×

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Protein purified on 1/15/2024 and assayed on 1/17/2024.
Data uploaded by storquato and last updated on 2024-02-24 14:49:07.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	0.00E+00	0.00E+00
	B	48.7	0.00E+00	0.00E+00	0.00E+00
	C	46.2	0.00E+00	0.00E+00	0.00E+00
	D	42.3	1.83E-02	1.90E-02	1.88E-02
	E	37.7	5.10E-02	5.54E-02	5.56E-02
	F	33.9	6.04E-02	6.04E-02	6.11E-02
	G	31.4	5.96E-02	6.14E-02	6.11E-02
	H	30.0	5.99E-02	6.09E-02	6.21E-02

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Protein purified on 1/15/2024 and assayed on 1/18/2024.
Data uploaded by storquato and last updated on 2024-03-29 13:30:43.

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.