D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E187I

Expression Data

Yield (mg/mL): 0.398

Kinetic Assay Data

**slope of E187I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.01E-01	1.01E-01	1.03E-01
B	25.00	8.85E-02	7.95E-02	8.05E-02
C	8.33	6.25E-02	6.45E-02	6.75E-02
D	2.78	2.95E-02	2.85E-02	2.75E-02
E	0.93	1.35E-02	1.20E-02	1.25E-02
F	0.31	5.00E-03	5.00E-03	5.00E-03
G	0.10	2.00E-03	2.00E-03	2.00E-03
H	0.03	5.00E-04		5.00E-04

Yield (mg/mL): 0.398 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by NoahSmithUNF2024 and last updated on 2024-04-18 10:40:35.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		6.46E-01	7.20E-01
B	25.00	5.61E-01	5.75E-01	5.38E-01
C	8.33	4.44E-01	4.10E-01	3.84E-01
D	2.78	2.10E-01	2.44E-01	2.27E-01
E	0.93	9.55E-02	9.20E-02	9.90E-02
F	0.31	3.00E-02	3.50E-02	3.50E-02
G	0.10	1.00E-02	1.20E-02	1.25E-02
H	0.03	1.48E-16	1.48E-16	1.48E-16

Yield (mg/mL): 5.018 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by NoahSmithUNF2024 and last updated on 2024-04-18 11:21:02.

Design Data

Rosetta Score for E187I: 30.158

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.