D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H182S

Expression Data

Yield (mg/mL): 1.183

Kinetic Assay Data

**slope of H182S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00
C	8.33	7.75E-01	7.96E-01	7.48E-01
D	2.78	4.51E-01	4.79E-01	4.85E-01
E	0.93	2.03E-01	1.99E-01	1.91E-01
F	0.31	8.65E-02	7.55E-02	7.95E-02
G	0.10	2.85E-02	2.90E-02	2.40E-02
H	0.03	5.00E-04	5.00E-04

Yield (mg/mL): 1.183 Dilution Factor: 10×

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by ChrisR and last updated on 2024-04-18 13:13:14.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.20E-01	4.26E-01	4.42E-01
B	25.00	3.92E-01	3.94E-01	4.02E-01
C	8.33	3.65E-01	3.34E-01	3.31E-01
D	2.78	1.76E-01	1.68E-01	1.80E-01
E	0.93	8.95E-02	8.40E-02	9.25E-02
F	0.31	3.45E-02	3.25E-02	3.30E-02
G	0.10	1.20E-02	1.40E-02	1.25E-02
H	0.03	1.48E-16		1.48E-16

Yield (mg/mL): 3.859 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by ChrisR and last updated on 2024-04-18 13:17:45.

Design Data

Rosetta Score for H182S: 2.733

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.