D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E177A

Expression Data

Yield (mg/mL): 6.124

Kinetic Assay Data

**slope of E177A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.31E-01	5.92E-01	5.93E-01
B	25.00	4.38E-01	4.46E-01	4.14E-01
C	8.33	3.85E-01	3.39E-01	3.36E-01
D	2.78	2.00E-01	2.02E-01	1.82E-01
E	0.93	7.75E-02	8.10E-02	6.95E-02
F	0.31	2.85E-02	3.05E-02	2.65E-02
G	0.10	9.50E-03	1.00E-02	9.50E-03
H	0.03	1.48E-16	1.48E-16	1.48E-16

Yield (mg/mL): 6.124 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/4/2024.
Data uploaded by dudleym and last updated on 2024-04-18 11:20:54.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.27E-01
B	25.00	7.06E-01	7.15E-01	7.19E-01
C	8.33	5.07E-01	4.72E-01	4.64E-01
D	2.78	3.04E-01	2.95E-01	2.69E-01
E	0.93	1.37E-01	1.12E-01	1.32E-01
F	0.31	4.30E-02	4.35E-02	4.30E-02
G	0.10	1.55E-02	1.55E-02	1.45E-02
H	0.03	1.48E-16	1.48E-16

Yield (mg/mL): 6.54 Dilution Factor: 100×

Protein purified on 4/4/2024 and assayed on 4/4/2024.
Data uploaded by dudleym and last updated on 2024-04-18 11:20:45.

Design Data

Rosetta Score for E177A: 3.091

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.