D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E187A

Expression Data

Yield (mg/mL): 8.07

Kinetic Assay Data

**slope of E187A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	0.7335	0.798	0.712
C	8.33	0.5225	0.538	0.519
D	2.78	0.3025	0.2795	0.324
E	0.93	0.124	0.127	0.1215
F	0.31	0.0475	0.0455	0.047
G	0.10	0.018	0.016	0.017
H	0.03		0.0005	0.0005

Yield (mg/mL): 8.07 Dilution Factor: 100×

Protein purified on 4/4/24 and assayed on 4/11/24.
Data uploaded by cyazji and last updated on 2024-04-18 11:24:05.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.6405		0.615
B	25.00	0.5205	0.493	0.543
C	8.33	0.439	0.441	0.396
D	2.78	0.2265	0.2275	0.237
E	0.93	0.099	0.1	0.1005
F	0.31	0.0405	0.035	0.037
G	0.10	0.012	0.013	0.013
H	0.03	1.48E-16	1.48E-16

Yield (mg/mL): 6.194 Dilution Factor: 100×

Protein purified on 4/4/24 and assayed on 4/11/24.
Data uploaded by cyazji and last updated on 2024-04-18 11:27:29.

Design Data

Rosetta Score for E187A: 2.263

Citations

The unpublished data above were collected by students from University of North Florida. For inquiries, please contact Hannah Malcolm: <Hannah.malcolm@unf.edu>.