D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R246K

Yield (mg/mL): 0.4752

**slope of R246K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.64E-02	8.16E-02	8.99E-02
B	25.00	7.88E-02	7.82E-02	7.98E-02
C	8.33	7.96E-02	7.18E-02	7.76E-02
D	2.78	6.15E-02	4.98E-02	5.89E-02
E	0.93	4.68E-02	3.57E-02	4.65E-02
F	0.31	3.10E-02	3.12E-02	3.59E-02
G	0.10	2.20E-02	3.09E-02	2.60E-02
H	0.03	1.57E-02	1.75E-02	1.82E-02

Yield (mg/mL): 0.4752 Dilution Factor: 100×

Protein purified on 3/6/2024 and assayed on 3/13/2024.
Data uploaded by ghylton and last updated on 2024-03-13 12:41:39.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.12E-01	1.15E-01	1.18E-01
B	25.00	1.04E-01	1.14E-01	1.06E-01
C	8.33	7.14E-02	9.38E-02	9.75E-02
D	2.78	5.56E-02	7.15E-02	6.54E-02
E	0.93	4.07E-02	5.20E-02	4.04E-02
F	0.31	3.26E-02	2.88E-02	3.33E-02
G	0.10		2.82E-02	3.06E-02
H	0.03	1.64E-02	1.65E-02	2.10E-02

Yield (mg/mL): 0.6563 Dilution Factor: 100×

Protein purified on 3/6/2024 and assayed on 3/13/2024.
Data uploaded by FNegri and last updated on 2024-03-13 12:48:50.

Rosetta Score for R246K: -0.586

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.