Raw Data for BglB Variant F243H

Expression Data

SDS-PAGE
 1.239

Kinetic Assay Data

slope of F243H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.85E-02 9.47E-02 9.78E-02
B 25.00 8.82E-02 8.74E-02 8.49E-02
C 8.33 7.37E-02 8.07E-02 7.09E-02
D 2.78 5.31E-02 6.32E-02 5.85E-02
E 0.93 4.07E-02 5.05E-02 4.41E-02
F 0.31 3.53E-02 3.58E-02 3.33E-02
G 0.10 3.09E-02 3.34E-02 2.83E-02
H 0.03 2.20E-02 2.16E-02 2.18E-02
 1.239      100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by mgramdinger and last updated on 2024-03-14 12:28:55.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.33E-01 1.45E-01 1.37E-01
B 25.00 1.16E-01 1.22E-01 1.28E-01
C 8.33 8.53E-02 1.15E-01 1.00E-01
D 2.78 8.20E-02 7.32E-02
E 0.93 3.59E-02 3.81E-02
F 0.31 2.12E-02 3.45E-02 2.80E-02
G 0.10 1.84E-02 1.69E-02
H 0.03 1.41E-02 1.45E-02 1.63E-02
 0.8935      100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by sdube and last updated on 2024-03-14 12:27:51.



Design Data

2.79


Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.