D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F243H

Yield (mg/mL): 1.102

**slope of F243H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.99E-01	2.11E-01	2.06E-01
B	25.00	1.66E-01	1.60E-01	1.60E-01
C	8.33	1.24E-01	1.14E-01	1.22E-01
D	2.78	9.11E-02	7.66E-02	8.90E-02
E	0.93	5.79E-02	4.87E-02	6.03E-02
F	0.31	3.52E-02	4.54E-02	3.94E-02
G	0.10	3.07E-02	3.41E-02
H	0.03	1.91E-02	2.03E-02	2.26E-02

Yield (mg/mL): 1.102 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by hpettibone and last updated on 2024-03-28 10:47:32.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.33E-01	1.45E-01	1.37E-01
B	25.00	1.16E-01	1.22E-01	1.28E-01
C	8.33	8.53E-02	1.15E-01	1.00E-01
D	2.78		8.20E-02	7.32E-02
E	0.93	3.59E-02		3.81E-02
F	0.31	2.12E-02	3.45E-02	2.80E-02
G	0.10	1.84E-02	1.69E-02
H	0.03	1.41E-02	1.45E-02	1.63E-02

Yield (mg/mL): 0.8935 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by sdube and last updated on 2024-03-14 12:27:51.

Rosetta Score for F243H: 2.79

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.