D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H379Y

Yield (mg/mL): 0.8904

**slope of H379Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.14E-02	8.66E-02	7.63E-02
B	25.00	5.50E-02	5.79E-02	6.79E-02
C	8.33		4.31E-02	4.78E-02
D	2.78	2.75E-02	3.17E-02	3.04E-02
E	0.93
F	0.31
G	0.10	1.75E-02		1.80E-02
H	0.03	1.03E-02	7.25E-03

Yield (mg/mL): 0.8904 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by lkreipke and last updated on 2024-03-14 12:39:23.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.93E-02	9.87E-02	1.05E-01
B	25.00	7.53E-02	8.87E-02	9.44E-02
C	8.33	5.50E-02	6.94E-02	6.04E-02
D	2.78	3.67E-02	5.14E-02	4.23E-02
E	0.93	1.98E-02		2.12E-02
F	0.31	1.27E-02	1.66E-02	1.77E-02
G	0.10	9.55E-03	1.10E-02	1.21E-02
H	0.03	5.70E-03	6.80E-03	8.10E-03

Yield (mg/mL): 0.6486 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by ogallagher and last updated on 2024-03-28 11:13:14.

Data missing or deleted from the database.

**slope of H379Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.89E-03	8.36E-03
B	48.7	4.70E-03	6.05E-03	6.95E-03
C	46.2	3.75E-03		4.34E-03
D	42.3	3.32E-02	4.03E-02	3.34E-02
E	37.7	1.13E-01	1.35E-01	1.36E-01
F	33.9	1.15E-01	1.27E-01	1.31E-01
G	31.4	1.22E-01	1.34E-01	1.27E-01
H	30.0	1.03E-01	1.25E-01	1.29E-01

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by slneilson and last updated on 2024-04-11 12:35:48.

Rosetta Score for H379Y: -0.904

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.