D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N270W

Yield (mg/mL): 0.5653

**slope of N270W A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.23E-02	5.07E-02	6.25E-02
B	25.00	5.34E-02	5.02E-02	4.93E-02
C	8.33	4.73E-02	4.61E-02	3.68E-02
D	2.78	3.91E-02	2.83E-02	3.18E-02
E	0.93	1.46E-02	1.29E-02	1.23E-02
F	0.31	1.19E-02	1.31E-02	9.90E-03
G	0.10	4.95E-03	5.85E-03	5.45E-03
H	0.03	5.15E-03		5.30E-03

Yield (mg/mL): 0.5653 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by afiala and last updated on 2024-03-28 11:23:38.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.09E-01	1.18E-01	1.17E-01
B	25.00	1.02E-01	9.47E-02	9.77E-02
C	8.33	7.09E-02	7.87E-02	7.99E-02
D	2.78	4.20E-02	5.56E-02	5.33E-02
E	0.93	3.26E-02	2.96E-02	2.91E-02
F	0.31	1.49E-02	1.33E-02	1.76E-02
G	0.10	1.35E-02	1.68E-02	1.16E-02
H	0.03	1.28E-02	9.30E-03	9.30E-03

Yield (mg/mL): 0.9171 Dilution Factor: 100×

Protein purified on 3/7/2024 and assayed on 3/14/2024.
Data uploaded by ecohler and last updated on 2024-03-14 12:23:30.

Rosetta Score for N270W: 8.565

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.