D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T296C

Expression Data

Yield (mg/mL): 0.173

Kinetic Assay Data

Plate 114962776

**slope of T296C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.50E-02	1.42E-02	1.60E-02
B	18.75	1.03E-02	1.10E-02	1.04E-02
C	4.69	5.39E-03	5.30E-03	6.41E-03
D	1.17	2.36E-03	2.38E-03	2.95E-03
E	0.29	7.19E-04	7.41E-04
F	0.07	1.90E-04	2.02E-04	2.59E-04
G	0.02	7.52E-05	7.70E-05	9.82E-05
H	0.00	3.58E-05	4.06E-05	3.76E-05

Yield / A_{280 nm}: 0.173 Dilution Factor: 10×

Protein purified on 2003.01.20 and assayed on 2003.02.20.
Data uploaded by akelly98 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.17E-01	8.72E-02	1.18E-01
B	18.75	1.18E-01	1.23E-01	1.07E-01
C	4.69	7.02E-02	6.39E-02	6.70E-02
D	1.17	3.61E-02	2.71E-02	3.04E-02
E	0.29		8.27E-03	9.04E-03
F	0.07		2.07E-03	2.29E-03
G	0.02		5.04E-04	5.61E-04
H	0.00		1.19E-04	1.37E-04

Yield / A_{280 nm}: 1.456 Dilution Factor: 100×

Protein purified on 2/29/20 and assayed on 2003.01.20.
Data uploaded by akelly98 and last updated on 2022-06-27 07:04:03.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.