Raw Data for BglB Variant H379Y

Expression Data

SDS-PAGE
 0.4505

Kinetic Assay Data

slope of H379Y A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.09E-02 1.00E-02
B 25.00 9.10E-03 9.05E-03
C 8.33 8.60E-03
D 2.78 5.75E-03
E 0.93
F 0.31 3.40E-03
G 0.10 3.05E-03
H 0.03 2.55E-03
 0.4505      100×

Protein purified on 3/8/2024 and assayed on 3/15/2024.
Data uploaded by gtuckett and last updated on 2024-03-15 12:47:47.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.14E-02 8.25E-02
B 25.00 6.53E-02 6.43E-02 6.51E-02
C 8.33 5.04E-02 4.87E-02 5.03E-02
D 2.78 2.67E-02 3.22E-02 3.15E-02
E 0.93 1.39E-02 1.56E-02 1.56E-02
F 0.31 8.65E-03 8.60E-03 1.07E-02
G 0.10 5.30E-03 4.70E-03 4.85E-03
H 0.03 3.00E-03 2.95E-03 3.40E-03
 0.893      100×

Protein purified on 3/8/2024 and assayed on 3/15/2024.
Data uploaded by ktiban and last updated on 2024-03-15 12:37:44.


Design Data

-0.904

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.