D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R246K

Yield (mg/mL): 0.4184

**slope of R246K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.10E-02	3.89E-02	4.19E-02
B	25.00	4.04E-02	3.82E-02
C	8.33	2.85E-02	2.98E-02	3.59E-02
D	2.78	2.46E-02	1.81E-02
E	0.93	1.50E-02	9.85E-03	1.76E-02
F	0.31	7.75E-03	9.20E-03	1.01E-02
G	0.10	7.80E-03	6.75E-03
H	0.03	6.85E-03	8.80E-03

Yield (mg/mL): 0.4184 Dilution Factor: 100×

Protein purified on 3/8/2024 and assayed on 3/15/2024.
Data uploaded by malmeida and last updated on 2024-03-15 12:58:12.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.15E-01	1.04E-01	9.96E-02
B	25.00	9.77E-02	8.85E-02	8.98E-02
C	8.33	6.73E-02	7.37E-02	7.24E-02
D	2.78	4.90E-02	5.82E-02	5.83E-02
E	0.93	2.19E-02		2.10E-02
F	0.31	1.01E-02	2.18E-02	1.53E-02
G	0.10	1.16E-02	5.50E-03	9.55E-03
H	0.03	3.85E-03	1.72E-02	7.40E-03

Yield (mg/mL): 1.091 Dilution Factor: 100×

Protein purified on 3/8/2024 and assayed on 3/15/2024.
Data uploaded by afeuer and last updated on 2024-03-15 12:36:36.

Rosetta Score for R246K: -0.586

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.