D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W325Q

Yield (mg/mL): 0.2993

**slope of W325Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.52E-02	5.58E-02	6.97E-02
B	25.00	6.05E-02	5.34E-02	6.25E-02
C	8.33	5.93E-02	5.75E-02	7.01E-02
D	2.78	5.35E-02	4.31E-02	5.70E-02
E	0.93	3.74E-02	3.06E-02	4.17E-02
F	0.31	3.14E-02	2.99E-02	2.68E-02
G	0.10	2.13E-02	2.03E-02	2.28E-02
H	0.03	1.55E-02	1.97E-02	1.39E-02

Yield (mg/mL): 0.2993 Dilution Factor: 10×

Protein purified on 3/5/2024 and assayed on 3/12/2024.
Data uploaded by Sanchal and last updated on 2024-03-26 11:01:38.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.06E-01	1.17E-01	1.14E-01
B	25.00	1.03E-01	1.04E-01	1.07E-01
C	8.33	7.46E-02	9.81E-02	9.26E-02
D	2.78	5.68E-02	6.80E-02	6.43E-02
E	0.93	4.20E-02	3.93E-02	4.25E-02
F	0.31	2.45E-02	2.47E-02	3.20E-02
G	0.10	2.16E-02	1.88E-02	2.56E-02
H	0.03	1.07E-02	1.53E-02	1.39E-02

Yield (mg/mL): 0.7292 Dilution Factor: 100×

Protein purified on 3/5/2024 and assayed on 3/12/2024.
Data uploaded by pmallari and last updated on 2024-03-26 10:33:09.

Rosetta Score for W325Q: 3.903

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.