D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H223A

Expression Data

Yield (mg/mL): 4.088

Kinetic Assay Data

**slope of H223A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.47E-01	2.06E-01	1.98E-01
B	25.00		1.56E-01	1.50E-01
C	8.33	1.10E-01	1.04E-01	1.11E-01
D	2.78	4.78E-02	4.87E-02	5.02E-02
E	0.93	1.90E-02	1.80E-02	1.97E-02
F	0.31	6.80E-03	6.78E-03	6.90E-03
G	0.10	2.11E-03	2.36E-03	2.33E-03
H	0.03	6.90E-04	8.20E-04	7.20E-04

Yield (mg/mL): 4.088 Dilution Factor: 100×

Protein purified on 2/20/24 and assayed on 2/21/24.
Data uploaded by rtsimmon and last updated on 2024-04-03 13:16:29.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.61E-01	3.39E-01	2.65E-01
B	25.00	2.95E-01	2.89E-01	2.72E-01
C	8.33	2.04E-01	1.92E-01	2.00E-01
D	2.78	1.18E-01		1.04E-01
E	0.93	3.08E-02	3.42E-02	3.25E-02
F	0.31	1.51E-02	1.52E-02	1.35E-02
G	0.10	5.31E-03	4.90E-03	5.04E-03
H	0.03	1.71E-03	1.77E-03

Yield (mg/mL): 3.872 Dilution Factor: 100×

Protein purified on 2/20/24 and assayed on 2/21/24.
Data uploaded by rtsimmon and last updated on 2024-04-02 14:38:24.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.