Raw Data for BglB Variant H315N

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of H315N A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.65E-03	3.39E-04	1.09E-03
	B	48.7	4.75E-04	1.86E-04	8.90E-04
	C	46.2	3.00E-05		8.35E-04
	D	42.3	2.08E-02	2.49E-02	2.97E-02
	E	37.7	5.58E-02	6.18E-02	6.67E-02
	F	33.9	6.94E-02	6.51E-02	7.09E-02
	G	31.4	6.69E-02	6.23E-02	6.91E-02
	H	30.0	6.49E-02	5.86E-02	5.63E-02

Download these data

Protein purified on 4/3/2024 and assayed on 4/10/2024.
Data uploaded by cplee and last updated on 2024-04-10 12:39:27.

Reference Data for WT

slope of H315N A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.49E-04		0.00E+00
	B	48.7	1.24E-03	1.60E-03	4.00E-04
	C	46.2	3.71E-03	2.43E-03	8.85E-04
	D	42.3	2.35E-02	2.31E-02	2.38E-02
	E	37.7	7.45E-02	7.24E-02	6.37E-02
	F	33.9	6.90E-02	6.71E-02	6.10E-02
	G	31.4	6.61E-02	7.49E-02	6.29E-02
	H	30.0	6.73E-02	6.72E-02	6.35E-02

Download these data

Protein purified on 4/3/2024 and assayed on 4/10/2024.
Data uploaded by lbotelho and last updated on 2024-04-10 12:42:54.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.