Raw Data for BglB Variant H315N

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of H315N A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.41E-05	5.94E-04	8.37E-04
	B	48.7	8.03E-04	3.01E-03	0.00E+00
	C	46.2	7.75E-04		1.01E-03
	D	42.3	2.96E-02	2.53E-02	2.42E-02
	E	37.7	7.64E-02	8.49E-02	7.92E-02
	F	33.9	7.40E-02	8.44E-02	8.12E-02
	G	31.4	7.57E-02	8.01E-02	8.31E-02
	H	30.0	7.43E-02		7.83E-02

Download these data

Protein purified on 4/3/2024 and assayed on 4/10/2024.
Data uploaded by ohetherington and last updated on 2024-04-10 12:42:28.

Reference Data for WT

slope of H315N A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.49E-04		0.00E+00
	B	48.7	1.24E-03	1.60E-03	4.00E-04
	C	46.2	3.71E-03	2.43E-03	8.85E-04
	D	42.3	2.35E-02	2.31E-02	2.38E-02
	E	37.7	7.45E-02	7.24E-02	6.37E-02
	F	33.9	6.90E-02	6.71E-02	6.10E-02
	G	31.4	6.61E-02	7.49E-02	6.29E-02
	H	30.0	6.73E-02	6.72E-02	6.35E-02

Download these data

Protein purified on 4/3/2024 and assayed on 4/10/2024.
Data uploaded by lbotelho and last updated on 2024-04-10 12:42:54.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.