Raw Data for BglB Variant R246K

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of R246K A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.09E-03	1.86E-03	6.54E-04
	B	48.7	1.09E-03	1.70E-03
	C	46.2		1.41E-03	1.15E-03
	D	42.3	8.91E-05	1.68E-03	6.44E-04
	E	37.7	2.02E-02	1.57E-02	1.93E-02
	F	33.9	4.96E-02	4.52E-02	4.75E-02
	G	31.4	5.45E-02	4.95E-02	5.29E-02
	H	30.0	5.28E-02	5.38E-02	5.24E-02

Download these data

Protein purified on 4/3/2024 and assayed on 4/10/2024.
Data uploaded by ookeeffe and last updated on 2024-04-10 12:41:08.

Reference Data for WT

slope of R246K A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.32E-03	1.27E-03	1.60E-03
	B	48.7	3.32E-03	2.45E-03	2.73E-03
	C	46.2		3.55E-03	3.24E-03
	D	42.3	2.84E-02	3.08E-02	2.33E-02
	E	37.7	9.12E-02	9.88E-02	7.84E-02
	F	33.9	9.93E-02	9.78E-02	8.68E-02
	G	31.4	9.26E-02	9.26E-02	7.45E-02
	H	30.0	8.31E-02	9.76E-02	6.97E-02

Download these data

Protein purified on 4/3/2024 and assayed on 4/10/2024.
Data uploaded by gsbennett and last updated on 2024-04-10 12:58:27.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.