Raw Data for BglB Variant H315N

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of H315N A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.79E-03	3.04E-04	2.71E-03
	B	48.7		1.09E-03	1.23E-03
	C	46.2	1.87E-03		2.19E-03
	D	42.3	1.69E-02	1.88E-02	1.93E-02
	E	37.7	5.08E-02	5.79E-02	6.23E-02
	F	33.9	5.66E-02	5.73E-02	6.41E-02
	G	31.4	4.27E-02	5.95E-02	5.45E-02
	H	30.0	3.44E-02	4.99E-02	6.03E-02

Download these data

Protein purified on 3/6/2024 and assayed on 4/10/2024.
Data uploaded by cfremming and last updated on 2024-04-10 12:48:04.

Reference Data for WT

slope of H315N A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.49E-04		0.00E+00
	B	48.7	1.24E-03	1.60E-03	4.00E-04
	C	46.2	3.71E-03	2.43E-03	8.85E-04
	D	42.3	2.35E-02	2.31E-02	2.38E-02
	E	37.7	7.45E-02	7.24E-02	6.37E-02
	F	33.9	6.90E-02	6.71E-02	6.10E-02
	G	31.4	6.61E-02	7.49E-02	6.29E-02
	H	30.0	6.73E-02	6.72E-02	6.35E-02

Download these data

Protein purified on 4/3/2024 and assayed on 4/10/2024.
Data uploaded by lbotelho and last updated on 2024-04-10 12:42:54.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.