Raw Data for BglB Variant F243H

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of F243H A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	0.00E+00	0.00E+00
	B	48.7	2.80E-04		1.28E-04
	C	46.2	5.92E-04		4.07E-04
	D	42.3	5.38E-02	6.11E-02	6.10E-02
	E	37.7	1.24E-01	1.23E-01	1.24E-01
	F	33.9	1.22E-01	1.13E-01	1.21E-01
	G	31.4	1.18E-01	1.22E-01	1.20E-01
	H	30.0	1.11E-01	1.19E-01	1.17E-01

Download these data

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by hpettibone and last updated on 2024-04-11 12:11:59.

Reference Data for WT

slope of F243H A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.11E-03	1.58E-03
	B	48.7	9.06E-04	5.57E-03	7.50E-03
	C	46.2	5.14E-03	6.14E-03	4.86E-03
	D	42.3	1.74E-02	2.12E-02	2.01E-02
	E	37.7	4.06E-02	4.31E-02	4.87E-02
	F	33.9	3.77E-02	4.31E-02	4.44E-02
	G	31.4	3.97E-02	4.08E-02	4.19E-02
	H	30.0	3.82E-02	4.00E-02	3.92E-02

Download these data

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by nmansoori and last updated on 2024-04-11 12:11:46.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.