Raw Data for BglB Variant F243H

Expression Data

SDS-PAGE
 not reported

Thermostability Assay Data

slope of F243H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 3.65E-04 0.00E+00
B 48.7 3.85E-03 4.10E-03
C 46.2 3.55E-03 4.09E-03 2.26E-03
D 42.3 7.22E-02 6.71E-02 6.12E-02
E 37.7 1.24E-01 1.35E-01 1.25E-01
F 33.9 1.18E-01 1.14E-01 1.24E-01
G 31.4 1.11E-01 1.14E-01 1.11E-01
H 30.0 1.10E-01 1.06E-01 1.11E-01

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by elarson and last updated on 2024-04-11 12:17:24.


Reference Data for WT

slope of F243H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 1.11E-03 1.58E-03
B 48.7 9.06E-04 5.57E-03 7.50E-03
C 46.2 5.14E-03 6.14E-03 4.86E-03
D 42.3 1.74E-02 2.12E-02 2.01E-02
E 37.7 4.06E-02 4.31E-02 4.87E-02
F 33.9 3.77E-02 4.31E-02 4.44E-02
G 31.4 3.97E-02 4.08E-02 4.19E-02
H 30.0 3.82E-02 4.00E-02 3.92E-02

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by nmansoori and last updated on 2024-04-11 12:11:46.



Design Data

2.79


Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.