Raw Data for BglB Variant F243H

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of F243H A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	3.34E-03	1.78E-03	1.59E-04
	B	48.7	1.12E-03		2.35E-03
	C	46.2	4.06E-03	2.41E-03	5.31E-03
	D	42.3	4.34E-02	4.15E-02	5.13E-02
	E	37.7	8.12E-02	7.63E-02	9.49E-02
	F	33.9	8.85E-02	9.31E-02	9.38E-02
	G	31.4	8.38E-02	7.72E-02	8.24E-02
	H	30.0	8.40E-02	8.25E-02	8.37E-02

Download these data

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by ocrowley and last updated on 2024-04-11 12:22:06.

Reference Data for WT

slope of F243H A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.11E-03	1.58E-03
	B	48.7	9.06E-04	5.57E-03	7.50E-03
	C	46.2	5.14E-03	6.14E-03	4.86E-03
	D	42.3	1.74E-02	2.12E-02	2.01E-02
	E	37.7	4.06E-02	4.31E-02	4.87E-02
	F	33.9	3.77E-02	4.31E-02	4.44E-02
	G	31.4	3.97E-02	4.08E-02	4.19E-02
	H	30.0	3.82E-02	4.00E-02	3.92E-02

Download these data

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by nmansoori and last updated on 2024-04-11 12:11:46.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.