Raw Data for BglB Variant N270W

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of N270W A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	2.37E-03		2.70E-03
	B	48.7	6.29E-03	5.51E-03	5.16E-03
	C	46.2	2.26E-03	2.08E-03
	D	42.3	1.30E-02	3.60E-03	9.72E-03
	E	37.7	5.04E-02	4.47E-02	4.25E-02
	F	33.9	5.32E-02	5.23E-02	5.81E-02
	G	31.4	4.95E-02	5.18E-02	6.10E-02
	H	30.0	5.09E-02	5.51E-02	5.26E-02

Download these data

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by sliu and last updated on 2024-04-11 12:27:58.

Reference Data for WT

slope of N270W A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.00E+00	1.22E-03	5.98E-04
	B	48.7	8.82E-04	1.73E-03	3.40E-03
	C	46.2	3.84E-03	5.18E-03	7.96E-03
	D	42.3	6.51E-02	6.45E-02	6.06E-02
	E	37.7	1.18E-01	1.34E-01	1.15E-01
	F	33.9	1.21E-01	1.20E-01	1.18E-01
	G	31.4	1.18E-01	1.21E-01	1.11E-01
	H	30.0	1.13E-01	1.18E-01	1.10E-01

Download these data

Protein purified on 4/4/2024 and assayed on 4/11/2024.
Data uploaded by mswhite and last updated on 2024-04-11 12:19:51.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.