Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 5.999

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.88E-01	2.81E-01	3.04E-01
	B	25.00	3.07E-01	2.94E-01	3.06E-01
	C	8.33	2.70E-01	2.74E-01	2.57E-01
	D	2.78	2.93E-01	2.69E-01	2.65E-01
	E	0.93	1.51E-01	1.45E-01	1.29E-01
	F	0.31	4.97E-02	4.89E-02	5.37E-02
	G	0.10	1.94E-02	2.07E-02
	H	0.03	0	0

Yield (mg/mL): 5.999     Dilution Factor:  100×

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Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by ivismesa and last updated on 2024-04-12 06:52:51.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0	0	0
	B	48.3		7.50E-04	6.00E-04
	C	45.7	7.50E-04	9.00E-04	1.05E-03
	D	42.4		4.50E-04	6.00E-04
	E	37.7	2.31E-01	2.25E-01	2.00E-01
	F	33.6	2.01E-01	2.11E-01	2.02E-01
	G	31.3	2.29E-01	2.30E-01	2.31E-01
	H	30.0	2.41E-01	2.05E-01	2.10E-01

Download these data

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by ivismesa and last updated on 2024-04-12 07:33:14.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.