D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E21Y

Expression Data

Yield (mg/mL): 4.452

Kinetic Assay Data

**slope of E21Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.60E-01	2.66E-01	2.75E-01
B	25.00	2.12E-01	2.22E-01	2.13E-01
C	8.33	1.75E-01	1.97E-01	1.87E-01
D	2.78	1.41E-01	1.49E-01	1.57E-01
E	0.93	1.01E-01	1.13E-01	1.11E-01
F	0.31	4.43E-02	4.25E-02	5.10E-02
G	0.10	1.58E-02	1.97E-02	1.62E-02
H	0.03		6.00E-04	6.00E-04

Yield (mg/mL): 4.452 Dilution Factor: 100×

Protein purified on 3/22/2024 and assayed on 4/12/2024.
Data uploaded by marcelaafanador and last updated on 2024-04-12 06:56:33.

Thermostability Assay Data

**slope of E21Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	9.00E-04	7.50E-04
B	48.3	6.00E-04	6.00E-04
C	45.7	6.00E-04		6.00E-04
D	42.4	1.20E-02	1.31E-02	1.17E-02
E	37.7	1.26E-01	1.22E-01	1.33E-01
F	33.6	1.29E-01	1.21E-01	1.17E-01
G	31.3	1.30E-01	1.30E-01	1.19E-01
H	30.0	1.36E-01	1.32E-01	1.25E-01

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by marcelaafanador and last updated on 2024-04-12 07:16:50.

Reference Data for WT

**slope of E21Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3	0		0
C	45.7	7.50E-04	6.00E-04	6.00E-04
D	42.4	9.15E-03	1.01E-02
E	37.7	9.41E-02	8.46E-02	8.13E-02
F	33.6	1.05E-01	9.66E-02	8.72E-02
G	31.3	1.05E-01	1.10E-01	9.33E-02
H	30.0	1.05E-01	1.02E-01	1.04E-01

Protein purified on 3/22/2024 and assayed on 4/29/2024.
Data uploaded by marcelaafanador and last updated on 2024-04-12 07:22:39.

Design Data

Rosetta Score for E21Y: 27.055

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.