Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 3.952

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	2.33E-01	2.28E-01	2.31E-01
	B	25.00	1.98E-01	1.96E-01	1.92E-01
	C	8.33	1.58E-01	1.49E-01	1.55E-01
	D	2.78	9.36E-02	9.51E-02	8.90E-02
	E	0.93	4.25E-02	3.98E-02	3.93E-02
	F	0.31	1.55E-02	1.46E-02	1.64E-02
	G	0.10	6.00E-03	5.55E-03	5.70E-03
	H	0.03	4.50E-04		4.50E-04

Yield (mg/mL): 3.952     Dilution Factor:  100×

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Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by sydneydevi and last updated on 2024-04-19 09:09:48.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.50E-04	7.50E-04	6.00E-04
	B	48.3		0	0
	C	45.7	7.50E-04	7.50E-04	7.50E-04
	D	42.4	1.52E-02	1.29E-02	1.29E-02
	E	37.7	9.62E-02	9.51E-02	9.56E-02
	F	33.6	1.07E-01	1.10E-01	1.05E-01
	G	31.3	1.07E-01	1.05E-01	9.95E-02
	H	30.0	1.09E-01	1.13E-01	9.27E-02

Download these data

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by sydneydevi and last updated on 2024-04-12 07:08:31.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.