Raw Data for BglB Variant F397A

Expression Data

SDS-PAGE
 0.6808

Kinetic Assay Data

slope of F397A A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.95E-03 6.00E-03
B 25.00 4.50E-03 4.20E-03 4.50E-03
C 8.33 3.90E-03 3.00E-03 3.30E-03
D 2.78 2.70E-03 2.55E-03 1.95E-03
E 0.93 1.35E-03 1.65E-03 1.65E-03
F 0.31 9.00E-04 1.05E-03 9.00E-04
G 0.10 6.00E-04 7.50E-04 6.00E-04
H 0.03 9.00E-04 4.50E-04 6.00E-04
 0.6808      100×

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by Yuliana and last updated on 2024-04-12 11:55:12.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.61E-01 4.48E-01 4.76E-01
B 25.00 3.57E-01 3.59E-01 3.85E-01
C 8.33 2.74E-01 2.63E-01 2.94E-01
D 2.78 1.60E-01 1.74E-01 1.74E-01
E 0.93 7.23E-02 7.49E-02 7.34E-02
F 0.31 2.67E-02 2.85E-02 2.87E-02
G 0.10 9.60E-03 9.75E-03 9.15E-03
H 0.03 0 0 0
 6.655      100×

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by Dominic and last updated on 2024-04-19 13:41:56.


Thermostability Assay Data

slope of F397A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00E+00 0.00E+00 0.00E+00
B 48.3
C 45.7 0.00E+00 0.00E+00
D 42.4 0.00E+00 0.00E+00
E 37.7 7.50E-04 6.00E-04 7.50E-04
F 33.6 6.00E-04 7.50E-04 6.00E-04
G 31.6 6.00E-04 7.50E-04
H 30.0 7.50E-04 7.50E-04

Protein purified on and assayed on .
Data uploaded by Yuliana and last updated on 2024-04-18 12:04:58.


Reference Data for WT

slope of F397A A420 nm (min−1)
T (°C) 1 2 3
A 50.0 7.50E-04 7.50E-04
B 48.3 7.50E-04 7.50E-04 7.50E-04
C 45.7 0 0 0
D 42.4 1.43E-02 1.43E-02
E 37.7 1.41E-01 1.55E-01 1.78E-01
F 33.6 1.98E-01 1.69E-01
G 31.3 1.52E-01 1.45E-01 1.67E-01
H 30.0 1.36E-01 1.58E-01 1.50E-01

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by Dominic and last updated on 2024-04-12 12:07:41.


Design Data

6.697

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.