Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 6.655

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	4.61E-01	4.48E-01	4.76E-01
	B	25.00	3.57E-01	3.59E-01	3.85E-01
	C	8.33	2.74E-01	2.63E-01	2.94E-01
	D	2.78	1.60E-01	1.74E-01	1.74E-01
	E	0.93	7.23E-02	7.49E-02	7.34E-02
	F	0.31	2.67E-02	2.85E-02	2.87E-02
	G	0.10	9.60E-03	9.75E-03	9.15E-03
	H	0.03	0	0	0

Yield (mg/mL): 6.655     Dilution Factor:  100×

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Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by Dominic and last updated on 2024-04-19 13:41:56.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	7.50E-04		7.50E-04
	B	48.3	7.50E-04	7.50E-04	7.50E-04
	C	45.7	0	0	0
	D	42.4	1.43E-02		1.43E-02
	E	37.7	1.41E-01	1.55E-01	1.78E-01
	F	33.6		1.98E-01	1.69E-01
	G	31.3	1.52E-01	1.45E-01	1.67E-01
	H	30.0	1.36E-01	1.58E-01	1.50E-01

Download these data

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by Dominic and last updated on 2024-04-12 12:07:41.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.