Raw Data for BglB Variant F397N

Expression Data

SDS-PAGE
 0.7082

Kinetic Assay Data

slope of F397N A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.59E-02 1.56E-02
B 25.00 1.41E-02 1.37E-02 1.32E-02
C 8.33 9.45E-03 9.30E-03 1.07E-02
D 2.78 3.00E-03 3.30E-03 3.00E-03
E 0.93 1.50E-03 1.50E-03 1.50E-03
F 0.31 1.05E-03 7.50E-04 9.00E-04
G 0.10 6.00E-04 7.50E-04 6.00E-04
H 0.03 0 0
 0.7082      100×

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by aledo010 and last updated on 2024-04-12 11:54:22.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.4696 0.4383 0.3891
B 25.00 0.3207 0.3229 0.3213
C 8.33 0.2715 0.2753 0.2652
D 2.78 0.09405 0.09495 0.0951
E 0.93 0.0393 0.03795 0.0417
F 0.31 0.0111 0.01425 0.01365
G 0.10 0.0051 0.00525 0.00525
H 0.03 0.0005998 0.0006 0.00075
 5.92      100×

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by luzsarrazola and last updated on 2024-04-12 11:54:25.


Thermostability Assay Data

slope of F397N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04
B 48.3 6.00E-04 7.50E-04
C 45.7 7.50E-04 6.00E-04 6.00E-04
D 42.4 1.05E-03 9.00E-04 7.50E-04
E 37.7 1.05E-03 1.20E-03 1.05E-03
F 33.6 1.65E-03 1.95E-03 1.65E-03
G 31.3 2.55E-03 2.40E-03 3.00E-03
H 30.0 2.70E-03 2.70E-03 2.55E-03

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by aledo010 and last updated on 2024-04-18 11:52:10.


Reference Data for WT

slope of F397N A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0006 0.0006 0.0006
B 48.3 0.00075 0.0006 0.00045
C 45.7 0.00105 0.00105
D 42.4 0.01545 0.01575 0.0159
E 37.7 0.141 0.1411 0.1223
F 33.6 0.1557 0.1583 0.1587
G 31.3 0.1451 0.1639 0.1491
H 30.0 0.1203 0.1238

Protein purified on 3/22/24 and assayed on 3/29/24.
Data uploaded by luzsarrazola and last updated on 2024-04-12 12:01:18.


Design Data

6.029

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.