Raw Data for BglB Variant N270W

Expression Data

Yield (mg/mL): not reported

Thermostability Assay Data

slope of N270W A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.32E-02	1.50E-02	1.46E-02
	B	48.7		9.77E-03	1.04E-02
	C	46.2	1.06E-02	9.61E-03
	D	42.3	1.48E-02		1.64E-02
	E	37.7	5.96E-02	5.20E-02	5.76E-02
	F	33.9	6.93E-02	6.93E-02	7.13E-02
	G	31.4	7.32E-02	6.93E-02	6.71E-02
	H	30.0	6.59E-02	6.74E-02	6.76E-02

Download these data

Protein purified on 4/5/2024 and assayed on 4/12/2024.
Data uploaded by skoskinen and last updated on 2024-04-12 12:29:00.

Reference Data for WT

slope of N270W A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	1.05E-02	1.13E-02	1.38E-02
	B	48.7	8.86E-03	9.88E-03	1.19E-02
	C	46.2		1.15E-02	1.05E-02
	D	42.3	4.43E-02	4.77E-02	4.43E-02
	E	37.7	8.04E-02	7.36E-02	7.29E-02
	F	33.9	6.92E-02	6.98E-02	7.33E-02
	G	31.4	7.29E-02	7.00E-02	7.26E-02
	H	30.0	6.66E-02	7.00E-02	6.77E-02

Download these data

Protein purified on 4/5/2024 and assayed on 4/12/2024.
Data uploaded by mblamb and last updated on 2024-04-12 12:15:48.

Design Data

Citations

The unpublished data above were collected by students from Smith College. For inquiries, please contact Samantha Torquato: <storquat@smith.edu>.